BV treatment does not significantly reduce the aggregation of SOD1 protein. (A) Cells were transiently transfected with a WT or G85R hSOD1-GFP construct for 24 hrs and then treated with 2.5 μg/ml BV or equal volume of saline for 24 hrs. At 48 hrs post-transfection, the cells were fixed and immunofluorescent staining was performed with an anti-GFP primary antibody. GFP-SOD1 aggregation was observed under a fluorescence microscope with fluoromount containing DAPI. Arrowheads indicate scattered filamentous aggregates, and arrows indicate condensed aggregates. (B) Quantification of A. The values shown are the means ± SEM of data obtained from three independent experiments. (bars, 25 μm.) * p < 0.05, ** p < 0.01. (C) Cells transfected with a WT or G85R hSOD1-GFP for 24 hrs treated with 2.5 μg/ml BV or equal volume of saline for 24 hrs. Cell viability was measured with MTT assay.