Figure 9

Bcl-2, p53 expression and TNF-α-induced NF-κB translocation in CACF-treated cells. (A) A375 cells were treated with various concentrations of CACF for 24 h. Cells were harvested and lysed for Western blot analysis. Membranes were probed with anti-Bcl-2, anti-p53 or anti-β-actin antibodies. Data were representative of two independent experiments. (B) A375 cells were pretreated with various concentrations of CACF prior to stimulation with 10 ng/ml TNF-α for 30 min. Cells were fixed, stained for NF-κB and visualized using HSC. (C) Bar chart showing the average fluorescence intensities of nuclear NF-κB in control/treated A375 cells in the absence/presence of TNF-α.