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Figure 4 | BMC Complementary and Alternative Medicine

Figure 4

From: Potentiation of neuritogenic activity of medicinal mushrooms in rat pheochromocytoma cells

Figure 4

Morphology of PC-12 cells in different treatment groups. PC-12 cells were exposed to U0126 (10 μM), PD98059 (40 μM) and LY294002 (30 μM) for 1 h before the treatment of aqueous extracts. (a) to (d) negative control (complete F-12 K medium), (e) to (h) positive control (NGF; 50 ng/ml), (i) to (l) positive control (H. erinaceus (HE); 50 μg/ml), (m) to (p) 75 μg/ml of G. lucidum (GL), (q) to (t) 50 μg/ml of G. neo-japonicum (GNJ), (u) to (x) 75 μg/ml of G. frondosa (GF). Nuclei stained blue and neurofilaments stained green. Scale bars represent 50 μM. Arrows indicate neurite outgrowth.

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