Figure 2

TLJN and geniposide rescue SH-SY5Y cells following formaldehyde treatment. SH-SY5Y cells were grown (1×10⁴) in 96-well plates overnight, and the culture medium was then replaced with serum-free DMEM in the presence of 0.12 mM formaldehyde, supplemented with TLJN (containing 100 μM geniposide and 10 μM ginsenoside Rg1), 100 μM geniposide, or 10 μM ginsenoside Rg1. Cell viability was evaluated using the cell counting kit-8 after a 24h incubation. Cells cultured without formaldehyde or drugs were used as a control ( A ). Changes in SH-SY5Y cell viability following treatment with different concentrations of geniposide (250, 100, 25 and 2.5 μM) in the presence of formaldehyde for 24 h ( B ) were detected. Data are expressed as the mean ± SD from at least three independent experiments. *P < 0.05, **P < 0.01 vs. formaldehyde-only treated group.