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Table 2 Biochemical properties of C. splendens polysaccharide fractions

From: Immunomodulatory activity of polysaccharides isolated from Clerodendrum splendens: Beneficial effects in experimental autoimmune encephalomyelitis

Fractionation step & Fraction name M.W. (kDa) Type II Arabino-galactan Polyphenol content (%) Methoxyl content (Mol %) DM (%)a
DEAE cellulose Diaion HP-20 Sepharose 6B      
CSP-A CSP-AB - 13.2 Negative 9.3 ± 0.29 1.0 ± 0.04 81
CSP-AU - 24.5 Negative 8.8 ± 0.25 0.9 ± 0.04 16
  CSP-AU1 38.5 Positive 7.6 ± 0.22 0.9 ± 0.04 30
CSP-AU2 8.6 Negative 8.1 ± 0.14 1.3 ± 0.03 N.D.
CSP-N CSP-NB - 12.0 Negative 0.9 ± 0.03 1.1 ± 0.01 67
CSP-NU - 21.7 Positive 0.5 ± 0.01 1.0 ± 0.02 32
  CSP-NU1 60.6 Positive 0.2 ± 0.02 N.D. N.D.
   CSP-NU2 5.5 Negative 6.5 ± 0.14 N.D. N.D.
  1. aThe degree of methyl esterification (DM) was calculated as the ratio of the molar amount of methanol to the molar amount of uronic acids (galacturonic and glucuronic acids) and is expressed as %. N.D. not determined.