|
Fractionation step & Fraction name
|
M.W. (kDa)
|
Type II Arabino-galactan
|
Polyphenol content (%)
|
Methoxyl content (Mol %)
|
DM (%)a
|
|---|
|
DEAE cellulose
|
Diaion HP-20
|
Sepharose 6B
| | | | | |
|---|
|
CSP-A
|
CSP-AB
|
-
|
13.2
|
Negative
|
9.3 ± 0.29
|
1.0 ± 0.04
|
81
|
|
CSP-AU
|
-
|
24.5
|
Negative
|
8.8 ± 0.25
|
0.9 ± 0.04
|
16
|
| |
CSP-AU1
|
38.5
|
Positive
|
7.6 ± 0.22
|
0.9 ± 0.04
|
30
|
|
CSP-AU2
|
8.6
|
Negative
|
8.1 ± 0.14
|
1.3 ± 0.03
|
N.D.
|
|
CSP-N
|
CSP-NB
|
-
|
12.0
|
Negative
|
0.9 ± 0.03
|
1.1 ± 0.01
|
67
|
|
CSP-NU
|
-
|
21.7
|
Positive
|
0.5 ± 0.01
|
1.0 ± 0.02
|
32
|
| |
CSP-NU1
|
60.6
|
Positive
|
0.2 ± 0.02
|
N.D.
|
N.D.
|
| | |
CSP-NU2
|
5.5
|
Negative
|
6.5 ± 0.14
|
N.D.
|
N.D.
|
-
aThe degree of methyl esterification (DM) was calculated as the ratio of the molar amount of methanol to the molar amount of uronic acids (galacturonic and glucuronic acids) and is expressed as %. N.D. not determined.
