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Table 2 Biochemical properties of C. splendens polysaccharide fractions

From: Immunomodulatory activity of polysaccharides isolated from Clerodendrum splendens: Beneficial effects in experimental autoimmune encephalomyelitis

Fractionation step & Fraction name

M.W. (kDa)

Type II Arabino-galactan

Polyphenol content (%)

Methoxyl content (Mol %)

DM (%)a

DEAE cellulose

Diaion HP-20

Sepharose 6B

     

CSP-A

CSP-AB

-

13.2

Negative

9.3 ± 0.29

1.0 ± 0.04

81

CSP-AU

-

24.5

Negative

8.8 ± 0.25

0.9 ± 0.04

16

 

CSP-AU1

38.5

Positive

7.6 ± 0.22

0.9 ± 0.04

30

CSP-AU2

8.6

Negative

8.1 ± 0.14

1.3 ± 0.03

N.D.

CSP-N

CSP-NB

-

12.0

Negative

0.9 ± 0.03

1.1 ± 0.01

67

CSP-NU

-

21.7

Positive

0.5 ± 0.01

1.0 ± 0.02

32

 

CSP-NU1

60.6

Positive

0.2 ± 0.02

N.D.

N.D.

  

CSP-NU2

5.5

Negative

6.5 ± 0.14

N.D.

N.D.

  1. aThe degree of methyl esterification (DM) was calculated as the ratio of the molar amount of methanol to the molar amount of uronic acids (galacturonic and glucuronic acids) and is expressed as %. N.D. not determined.