Figure 5
Effect of Con A and LPS on GM-CSF production by human PBMCs. PBMCs were incubated for 24 hr with the indicated concentrations of Con A (○) or LPS (□). Cell-free supernatants were collected, and extracellular GM-CSF was quantified by ELISA. Values are the mean ± S.D. of triplicate samples from one experiment, which is representative of two independent experiments. Statistically significant differences (* p<0.05) between PBS-treated cells and cells treated with Con A or LPS are indicated.