Figure 2

Effect of Clerodendrum polysaccharide fractions on monocyte/macrophage TNF production. Murine J774.A1 macrophages (panels A-C), human MonoMac-6 cells (panels D-F), or PBMCs (panels D-F) were incubated for 24 hr with the indicated concentrations of CSP-NU (■, panels A, D, and G), CSP-AU (□, panels A, D, and G), CSP-NB (●, panels A, D, and G), CSP-AB (○, panels A, D, and G), CSP-AU1 (♦, panels B, E, and H), CSP-AU2 (◊, panels B, E, and H), CSP-NU1 (▲, panels C, F, and I), CSP-NU2 (Δ, panels C, F, and I), or 200 ng/ml LPS (, panels A, D, and G). Cell-free supernatants were collected, and extracellular TNF was quantified by ELISA. Values are the mean ± S.D. of triplicate samples from one experiment, which is representative of three independent experiments. Statistically significant differences (* p<0.05) between PBS-treated cells and cells treated with polysaccharide fractions or LPS are indicated.