Figure 12
Cytokine profile of splenic and lymph node (LN) cells isolated from PBS- or CSP AU1-treated EAE mice. Splenic and LN cells were prepared from mice at day 17 after immunization with MOG35-55 peptide. These cells (5 × 106 cell/ml) were restimulated with 10 μg/ml MOG35-55 for 4 days, cell-free supernatants were collected, and extracellular cytokines were quantified by ELISA. Mean cytokine concentrations from 3–5 cultures ± S.D. are shown. Statistically significant differences (* p<0.05; ** p<0.001) between cells, isolated from PBS- and CSP-AU1-treated EAE mice are indicated.