Effect of EESP on the expression of Bcl-2, Bax, Cyclin D1, CDK4, VEGF-A and VEGFR-2 in CRC mice. (A) The mRNA levels in tumor tissues from control and EESP-treated group were determined by RT-PCR. GAPDH was used as an internal control. The data of densitometric analysis were normalized to the mean mRNA expression of untreated control (100%). (B-D) The protein expression of Bcl-2, Bax, Cyclin D1, CDK4, VEGF-A and VEGFR-2 in tumor tissues was analyzed via immunohistochemical assay. The photographs are representative images taken at a magnification of 400 ×. Quantification of immunohistochemical assay was represented as percentage of positively-stained cells. Data shown are averages with S.D. (error bars) from 9 individual mouse in each group. *P< 0.01, versus controls.