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Table 7 Anti-hemolytic activity of extract and various fractions of M. royleanus leaves

From: Assessment of phytochemicals, antioxidant, anti-lipid peroxidation and anti-hemolytic activity of extract and various fractions of Maytenus royleanus leaves

Extract

Positive controla

Negative controlb

Optical densityc

 
   

Concentration μg/ml

 
   

10

50

100

200

250

 

0.41 ± 0.01

1.07 ± 0.02

     

Methanol extract

  

1.03 ± 0.02 a

0.94 ± 0.02 b

0.88 ± 0.04 b

0.84 ± 0.02 b

0.78 ± 0.03 b

 

0.41 ± 0.01

1.07 ± 0.02

     

n-hexane fraction

  

1.01 ± 0.03 a

0.98 ± 0.03 a

0.87 ± 0.02 b

0.82 ± 0.04 b

0.76 ± 0.04 b

 

0.41 ± 0.01

1.07 ± 0.02

     

Chloroform fraction

  

0.94 ± 0.04 b

0.76 ± 0.03 c

0.74 ± 0.03 c

0.68 ± 0.03 d

0.64 ± 0.03 c

 

0.41 ± 0.01

1.07 ± 0.02

     

Ethyl acetate fraction

  

1.03 ± 0.02 a

0.98 ± 0.04 a

0.96 ± 0.04 a

0.92 ± 0.04 a

0.85 ± 0.05 a

 

0.41 ± 0.01

1.07 ± 0.02

     

n-butanol fraction

  

0.87 ± 0.03c

0.74 ± 0.04 c

0.70 ± 0.04 c

0.65 ± 0.04 c

0.58 ± 0.04 d

 

0.41 ± 0.01

1.07 ± 0.02

     

Residual aqueous fraction

  

0.81 ± 0.02 d

0.76 ± 0.02 c

0.71 ± 0.02 c

0.64 ± 0.03 c

0.54 ± 0.03 e

  1. Each value is represented as mean ± SE (n = 3). Values in the same column followed by a different letter (a-e) are significantly different (p < 0.05).
  2. aWater replaced extract/fractions to serve as a positive control since this treatment results in 100% hemolysis.
  3. b Phosphate buffered saline replaced extract/fraction to serve as a negative control since this treatment results in 0% hemolysis.
  4. c Lower values are associated with increased cell lysis.