|
Extract
|
Positive controla
|
Negative controlb
|
Optical densityc
| |
|---|
| | | |
Concentration μg/ml
| |
|---|
| | | |
10
|
50
|
100
|
200
|
250
|
| |
0.41 ± 0.01
|
1.07 ± 0.02
| | | | | |
|
Methanol extract
| | |
1.03 ± 0.02 a
|
0.94 ± 0.02 b
|
0.88 ± 0.04 b
|
0.84 ± 0.02 b
|
0.78 ± 0.03 b
|
| |
0.41 ± 0.01
|
1.07 ± 0.02
| | | | | |
|
n-hexane fraction
| | |
1.01 ± 0.03 a
|
0.98 ± 0.03 a
|
0.87 ± 0.02 b
|
0.82 ± 0.04 b
|
0.76 ± 0.04 b
|
| |
0.41 ± 0.01
|
1.07 ± 0.02
| | | | | |
|
Chloroform fraction
| | |
0.94 ± 0.04 b
|
0.76 ± 0.03 c
|
0.74 ± 0.03 c
|
0.68 ± 0.03 d
|
0.64 ± 0.03 c
|
| |
0.41 ± 0.01
|
1.07 ± 0.02
| | | | | |
|
Ethyl acetate fraction
| | |
1.03 ± 0.02 a
|
0.98 ± 0.04 a
|
0.96 ± 0.04 a
|
0.92 ± 0.04 a
|
0.85 ± 0.05 a
|
| |
0.41 ± 0.01
|
1.07 ± 0.02
| | | | | |
|
n-butanol fraction
| | |
0.87 ± 0.03c
|
0.74 ± 0.04 c
|
0.70 ± 0.04 c
|
0.65 ± 0.04 c
|
0.58 ± 0.04 d
|
| |
0.41 ± 0.01
|
1.07 ± 0.02
| | | | | |
|
Residual aqueous fraction
| | |
0.81 ± 0.02 d
|
0.76 ± 0.02 c
|
0.71 ± 0.02 c
|
0.64 ± 0.03 c
|
0.54 ± 0.03 e
|
- Each value is represented as mean ± SE (n = 3). Values in the same column followed by a different letter (a-e) are significantly different (p < 0.05).
-
aWater replaced extract/fractions to serve as a positive control since this treatment results in 100% hemolysis.
-
b Phosphate buffered saline replaced extract/fraction to serve as a negative control since this treatment results in 0% hemolysis.
-
c Lower values are associated with increased cell lysis.
