Skip to main content

Table 7 Anti-hemolytic activity of extract and various fractions of M. royleanus leaves

From: Assessment of phytochemicals, antioxidant, anti-lipid peroxidation and anti-hemolytic activity of extract and various fractions of Maytenus royleanus leaves

Extract Positive controla Negative controlb Optical densityc  
    Concentration μg/ml  
    10 50 100 200 250
  0.41 ± 0.01 1.07 ± 0.02      
Methanol extract    1.03 ± 0.02 a 0.94 ± 0.02 b 0.88 ± 0.04 b 0.84 ± 0.02 b 0.78 ± 0.03 b
  0.41 ± 0.01 1.07 ± 0.02      
n-hexane fraction    1.01 ± 0.03 a 0.98 ± 0.03 a 0.87 ± 0.02 b 0.82 ± 0.04 b 0.76 ± 0.04 b
  0.41 ± 0.01 1.07 ± 0.02      
Chloroform fraction    0.94 ± 0.04 b 0.76 ± 0.03 c 0.74 ± 0.03 c 0.68 ± 0.03 d 0.64 ± 0.03 c
  0.41 ± 0.01 1.07 ± 0.02      
Ethyl acetate fraction    1.03 ± 0.02 a 0.98 ± 0.04 a 0.96 ± 0.04 a 0.92 ± 0.04 a 0.85 ± 0.05 a
  0.41 ± 0.01 1.07 ± 0.02      
n-butanol fraction    0.87 ± 0.03c 0.74 ± 0.04 c 0.70 ± 0.04 c 0.65 ± 0.04 c 0.58 ± 0.04 d
  0.41 ± 0.01 1.07 ± 0.02      
Residual aqueous fraction    0.81 ± 0.02 d 0.76 ± 0.02 c 0.71 ± 0.02 c 0.64 ± 0.03 c 0.54 ± 0.03 e
  1. Each value is represented as mean ± SE (n = 3). Values in the same column followed by a different letter (a-e) are significantly different (p < 0.05).
  2. aWater replaced extract/fractions to serve as a positive control since this treatment results in 100% hemolysis.
  3. b Phosphate buffered saline replaced extract/fraction to serve as a negative control since this treatment results in 0% hemolysis.
  4. c Lower values are associated with increased cell lysis.