Table 1 Showing primers and probe used
Gene name | Forward primer | Reverse primer | Probe |
|---|---|---|---|
Glutathione peroxidase | 5’-GGTGTTCCAGTGCGCAGAT-3’ | 5’-AGGGCTTCTATATCGGGTTCGA-3’ | FAM-CAGCAGGCGCTTTCGCACCA-TAMRA |
Glutathione reductase | 5’-TGAGCCGCCTGAACAACA-3’ | 5’-TTGCGTAGCCGTGGATGAC-3’ | FAM-CCAAAACAATTTAACCAAGTCCCACATCGA-TAMRA |
Glutathione s transferase -α | 5’-AATATGTCCCCCAGACCAAAGA-3’ | 5’-GGCAGGCAAGTACCGGTTT-3’ | FAM-CGCCTTGGCAAAAGACAGGACCA-TAMRA |
Glutamate-cysteine ligase | 5’CAGAGTATGGGAGTTACATGATTGAAG-3’ | 5’-TGTGTTGAACTCGGACATCGTT-3’ | FAM-ACACCTGGCCAGCCGTACGGAG-TAMRA |
Paraoxonase-1 | 5’-TGAGAGCTTCTATGCCACAAATG-3’ | 5’-CCATGACAGGCCCAAGTACA-3’ | FAM-TTTTGCTGACCCATACTTACGGTCCTGG-TAMRA |
Sulfiredoxin-1 | 5’-AATCCCCAACCCCTGACTTT-3’ | 5’-TGAACTGACCAGTGGAGACACAGT-3’ | FAM-ACAAGGTTTCTTCAGCCCCGGTGC-TAMRA |
GAPDH | 5'- TGGCCTCCAAGGAGTAAGAAAC -'3 | 5'- GGCCTCTCTCTTGCTCTCAGTATC -'3 | FAM- CTGGACCACCCAGCCCAGCAA-TAMRA |