Effects of TLBZT and 5-FU on angiogenesis. A, after three weeks treatment, angiogenesis and VEGF expression in CT26 carcinomas (3 tumors/group) were detected by immunohistochemistry and observed under microscope (×200). B, vessel density was determined via counting the number of the microvessels per high-power field (3 HPF/slide), and the MOD of VEGF (3 HPF/slide) were analyzed by Image-Pro Plus 6.0 software, and expressed as fold over control. C, VEGF expression in CT26 carcinomas were further verified by western blot. GAPDH was used as a loading control. **P<0.01, versus control group. ▲
P>0.05, versus control group.