Effects of TLBZT on cell senescence related gene expression. After three weeks treatment, p16 and p21, and RB phosphorylation in CT26 carcinomas (3 tumors/group) were detected by immunohistochemistry and observed under microscope (×200) (A). The p21, p16 and RB phosphorylation positive cells (3 HPF/slide) were counted by Image-Pro Plus 6.0 software, and expressed as mean ± SD (B). C, p16 and p21 expression and RB phosphorylation was further detected with western blot by specific antibody. GAPDH was used as a loading control. **P<0.01, versus control group.