Figure 2

Photomicrographs of histological changes of rat liver at magnification of 10×. (A) normal control showed well maintained histology of rat liver (−); (B) CCl₄ treated control rats showed lymphocyte infiltration (red arrow), fatty changes (blue arrow) and liver cell hypertrophy with congestion of hepatic sinusoids (green arrow) (+++); (C) Silymarin (100 mg/kg, p.o.) + CCl₄ (1.0 ml/kg in olive oil 1:1, i.p.) treated group showed scarce lymphocyte infiltration and preserved cell architecture (+); (D) TPW (400 mg/kg, p.o.) + CCl₄ (1.0 ml/kg in olive oil 1:1, i.p.) treated rat liver showed improved structure with mild lymphocyte infiltration (+). Scoring was performed as follows: –, no injury; +++, severe injury; ++, moderate injury; +, mild injury.