Parameter | Sex | Dose (mg/kg) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0 mg/kg | 1000 mg/kg | 2500 mg/kg | 5000 mg/kg | ||||||||||
13 wk | 26 wk | Recovery | 13 wk | 26 wk | Recovery | 13 wk | 26 wk | Recovery | 13wk | 26 wk | Recovery | ||
Body weight | Male | 486.2 ± 16.4 | 604.4 ± 70.0 | 641.3 ± 95.7 | 516.3 ± 101.3 | 634.6 ± 60.1 | 670.7 ± 65.7 | 482.3 ± 44.4 | 628.1 ± 67.7 | 660.4 ± 39.7 | 481.8 ± 39.5 | 637.8 ± 82.5 | 691.7 ± 63.8 |
Female | 291.3 ± 16.8 | 331.8 ± 27.2 | 338.9 ± 35.6 | 280.9 ± 17.0 | 333.4 ± 32.2 | 329.7 ± 34.7 | 266.0 ± 9.3 | 308.3 ± 25.7 | 318.4 ± 15.2 | 269.0 ± 36.4 | 322.2 ± 31.9 | 336.6 ± 29.5 | |
Brain | Male | 2.096 ± 0.064 | 2.240 ± 0.124 | 2.163 ± 0.103 | 2.214 ± 0.115 | 2.221 ± 0.095 | 2.191 ± 0.090 | 2.080 ± 0.117 | 2.281 ± 0.079 | 2.224 ± 0.092 | 2.092 ± 0.183 | 2.238 ± 0.089 | 2.261 ± 0.049 |
Female | 1.996 ± 0.066 | 2.021 ± 0.064 | 2.088 ± 0.096 | 1.947 ± 0.040 | 2.011 ± 0.064 | 2.028 ± 0.043 | 2.006 ± 0.046 | 2.008 ± 0.105 | 2.003 ± 0.063 | 1.985 ± 0.029 | 2.040 ± 0.060 | 2.029 ± 0.039 | |
% to body weight | Male | 0.431 ± .016 | 0.376 ± 0.054 | 0.344 ± 0.055 | 0.439 ± 0.067 | 0.352 ± 0.033 | 0.329 ± 0.034 | 0.434 ± 0.039 | 0.366 ± 0.036 | 0.338 ± 0.027 | 0.436 ± 0.043 | 0.356 ± 0.046 | 0.329 ± 0.030 |
Female | 0.687 ± 0.041 | 0.612 ± 0.041 | 0.621 ± 0.066 | 0.695 ± 0.045 | 0.608 ± 0.060 | 0.620 ± 0.065 | 0.754 ± 0.023* | 0.655 ± 0.057 | 0.630 ± 0.019 | 0.747 ± 0.082 | 0.638 ± 0.065 | 0.606 ± 0.051 | |
Thymus | Male | 0.297 ± 0.097 | 0.270 ± 0.069 | 0.262 ± 0.063 | 0.351 ± 0.170 | 0.219 ± 0.106 | 0.250 ± 0.092 | 0.313 ± 0.043 | 0.223 ± 0.084 | 0.200 ± 0.038 | 0.270 ± 0.048 | 0.262 ± 0.056 | 0.224 ± 0.059 |
Female | 0.367 ± 0.057 | 0.245 ± 0.073 | 0.187 ± 0.057 | 0.325 ± 0.041 | 0.232 ± 0.038 | 0.225 ± 0.045 | 0.283 ± 0.053 | 0.226 ± 0.061 | 0.214 ± 0.071 | 0.304 ± 0.091 | 0.231 ± 0.063 | 0.218 ± 0.023 | |
% to body weight | Male | 0.061 ± 0.018 | 0.044 ± 0.009 | 0.041 ± 0.008 | 0.065 ± 0.019 | 0.034 ± 0.013* | 0.037 ± 0.013 | 0.065 ± 0.011 | 0.035 ± 0.012 | 0.030 ± 0.005* | 0.056 ± 0.008 | 0.041 ± 0.008 | 0.032 ± 0.006 |
Female | 0.126 ± 0.017 | 0.074 ± 0.023 | 0.055 ± 0.014 | 0.116 ± 0.015 | 0.070 ± 0.010 | 0.069 ± 0.015 | 0.106 ± 0.018 | 0.073 ± 0.017 | 0.067 ± 0.021 | 0.112 ± 0.026 | 0.071 ± 0.014 | 0.065 ± 0.009 | |
Heart | Male | 1.381 ± 0.111 | 1.625 ± 0.204 | 1.653 ± 0.259 | 1.494 ± 0.193 | 1.710 ± 0.229 | 1.613 ± 0.225 | 1.408 ± 0.156 | 1.702 ± 0.169 | 1.749 ± 0.138 | 1.467 ± 0.093 | 1.699 ± 0.182 | 1.912 ± 0.182 |
Female | 0.832 ± 0.042 | 1.100 ± 0.101 | 1.046 ± 0.138 | 0.917 ± 0.094 | 1.089 ± 0.109 | 1.013 ± 0.091 | 0.905 ± 0.066 | 1.024 ± 0.091 | 0.986 ± 0.069 | 0.969 ± 0.102 | 1.098 ± 0.136 | 1.014 ± 0.068 | |
% to body weight | Male | 0.284 ± 0.014 | 0.270 ± 0.029 | 0.258 ± 0.004 | 0.294 ± 0.037 | 0.271 ± 0.039 | 0.240 ± 0.015* | 0.292 ± 0.021 | 0.272 ± 0.021 | 0.265 ± 0.020 | 0.306 ± 0.028 | 0.268 ± 0.016 | 0.277 ± 0.016 |
Female | 0.286 ± 0.012 | 0.333 ± 0.034 | 0.308 ± 0.010 | 0.326 ± 0.023* | 0.328 ± 0.027 | 0.308 ± 0.016 | 0.341 ± 0.036* | 0.334 ± 0.044 | 0.310 ± 0.016 | 0.362 ± 0.025** | 0.341 ± 0.032 | 0.302 ± 0.018 | |
Lung | Male | 1.782 ± 0.201 | 1.831 ± 0.271 | 1.845 ± 0.237 | 1.862 ± 0.779 | 1.876 ± 0.246 | 1.910 ± 0.094 | 1.528 ± 0.103* | 1.930 ± 0.088 | 1.916 ± 0.052 | 1.516 ± 0.066* | 1.898 ± 0.127 | 2.099 ± 0.322 |
Female | 1.210 ± 0.061 | 1.434 ± 0.110 | 1.409 ± 0.098 | 1.506 ± 0.538 | 1.449 ± 0.168 | 1.28 ± 0.062* | 1.210 ± 0.072 | 1.438 ± 0.166 | 1.419 ± 0.048 | 1.204 ± 0.192 | 1.443 ± 0.138 | 1.503 ± 0.152 | |
% to body weight | Male | 0.366 ± 0.038 | 0.304 ± 0.041 | 0.289 ± 0.025 | 0.354 ± 0.087 | 0.296 ± 0.034 | 0.287 ± 0.033 | 0.318 ± 0.021* | 0.310 ± 0.029 | 0.291 ± 0.016 | 0.316 ± 0.026 | 0.300 ± 0.028 | 0.303 ± 0.035 |
Female | 0.416 ± 0.024 | 0.434 ± 0.036 | 0.418 ± 0.043 | 0.537 ± 0.189 | 0.438 ± 0.064 | 0.394 ± 0.041 | 0.456 ± 0.037 | 0.466 ± 0.036 | 0.446 ± 0.025 | 0.447 ± 0.021 | 0.450 ± 0.048 | 0.448 ± 0.041 | |
Liver | Male | 11.492 ± 1.894 | 12.852 ± 1.753 | 14.019 ± 2.841 | 12.564 ± 3.570 | 13.450 ± 1.903 | 14.001 ± 1.859 | 11.882 ± 1.267 | 14.860 ± 2.256* | 15.212 ± 1.286 | 11.380 ± 0.892 | 16.043 ± 3.106* | 16.306 ± 1.798 |
Female | 6.885 ± 0.495 | 8.213 ± 1.184 | 7.582 ± 0.862 | 7.057 ± 0.719 | 8.522 ± 0.984 | 7.761 ± 1.048 | 6.920 ± 0.305 | 8.136 ± 0.742 | 7.659 ± 0.794 | 7.356 ± 1.218 | 8.733 ± 0.742 | 7.594 ± 0.724 | |
% to body weight | Male | 2.356 ± 0.312 | 2.125 ± 0.125 | 2.180 ± 0.190 | 2.407 ± 0.256 | 2.117 ± 0.187 | 2.084 ± 0.143 | 2.461 ± 0.062 | 2.363 ± 0.209** | 2.301 ± 0.061 | 2.366 ± 0.123 | 2.498 ± 0.193** | 2.357 ± 0.164 |
Female | 2.369 ± 0.223 | 2.481 ± 0.355 | 2.239 ± 0.151 | 2.510 ± 0.189 | 2.561 ± 0.237 | 2.351 ± 0.132 | 2.605 ± 0.169 | 2.644 ± 0.205 | 2.402 ± 0.171 | 2.728 ± 0.105* | 2.728 ± 0.300 | 2.258 ± 0.159 | |
Spleen | Male | 0.614 ± 0.042 | 0.799 ± 0.116 | 0.833 ± 0.115 | 0.639 ± 0.105 | 0.849 ± 0.110 | 0.795 ± 0.096 | 0.708 ± 0.166 | 0.810 ± 0.115 | 0.868 ± 0.133 | 0.673 ± 0.073 | 0.916 ± 0.101* | 0.859 ± 0.104 |
Female | 0.431 ± 0.023 | 0.618 ± 0.149 | 0.582 ± 0.108 | 0.460 ± 0.054 | 0.544 ± 0.074 | 0.553 ± 0.060 | 0.478 ± 0.035 | 0.574 ± 0.115 | 0.545 ± 0.035 | 0.446 ± 0.081 | 0.518 ± 0.080 | 0.543 ± 0.057 | |
% to body weight | Male | 0.126 ± 0.006 | 0.133 ± 0.022 | 0.130 ± 0.009 | 0.126 ± 0.021 | 0.134 ± 0.009 | 0.118 ± 0.007 | 0.147 ± 0.030 | 0.129 ± 0.009 | 0.131 ± 0.018 | 0.140 ± 0.015 | 0.145 ± 0.015 | 0.124 ± 0.009 |
Female | 0.148 ± 0.009 | 0.187 ± 0.046 | 0.172 ± 0.026 | 0.164 ± 0.020 | 0.164 ± 0.021 | 0.168 ± 0.013 | 0.180 ± 0.016** | 0.186 ± 0.033 | 0.171 ± 0.006 | 0.167 ± 0.028 | 0.162 ± 0.026 | 0.162 ± 0.018 | |
Kidney | Male | 2.832 ± 0.228 | 3.343 ± 0.322 | 3.406 ± 0.309 | 2.943 ± 0.433 | 3.421 ± 0.481 | 3.449 ± 0.451 | 2.766 ± 0.141 | 3.519 ± 0.316 | 3.856 ± 0.456 | 3.013 ± 0.428 | 3.622 ± 0.410 | 4.178 ± 0.503* |
Female | 1.655 ± 0.139 | 1.896 ± 0.164 | 1.868 ± 0.199 | 1.568 ± 0.152 | 1.932 ± 0.175 | 1.914 ± 0.255 | 1.605 ± 0.045 | 1.978 ± 0.175 | 1.892 ± 0.111 | 1.626 ± 0.192 | 2.025 ± 0.164 | 1.857 ± 0.167 | |
% to body weight | Male | 0.582 ± 0.031 | 0.557 ± 0.056 | 0.537 ± 0.060 | 0.577 ± 0.070 | 0.540 ± 0.072 | 0.513 ± 0.029 | 0.577 ± 0.058 | 0.563 ± 0.048 | 0.584 ± 0.062 | 0.624 ± 0.059 | 0.572 ± 0.059 | 0.603 ± 0.026 |
Female | 0.569 ± 0.052 | 0.574 ± 0.058 | 0.552 ± 0.031 | 0.558 ± 0.048 | 0.582 ± 0.054 | 0.581 ± 0.049 | 0.604 ± 0.027 | 0.643 ± 0.049** | 0.594 ± 0.024* | 0.606 ± 0.029* | 0.632 ± 0.056 | 0.552 ± 0.034 | |
Adrenal grand | Male | 0.068 ± 0.012 | 0.050 ± 0.006 | 0.062 ± 0.009 | 0.059 ± 0.013 | 0.056 ± 0.007 | 0.051 ± 0.005* | 0.062 ± 0.012 | 0.055 ± 0.009 | 0.057 ± 0.005 | 0.053 ± 0.009* | 0.056 ± 0.008 | 0.065 ± 0.010 |
Female | 0.066 ± 0.007 | 0.063 ± 0.009 | 0.067 ± 0.009 | 0.061 ± 0.009 | 0.063 ± 0.008 | 0.055 ± 0.006* | 0.066 ± 0.006 | 0.065 ± 0.012 | 0.064 ± 0.011 | 0.061 ± 0.008 | 0.069 ± 0.009 | 0.057 ± 0.010 | |
% to body weight | Male | 0.014 ± 0.002 | 0.008 ± 0.002 | 0.010 ± 0.001 | 0.012 ± 0.004 | 0.009 ± 0.001 | 0.008 ± 0.001* | 0.013 ± 0.003 | 0.009 ± 0.002 | 0.009 ± 0.001 | 0.011 ± 0.002 | 0.009 ± 0.002 | 0.009 ± 0.001 |
Female | 0.023 ± 0.002 | 0.019 ± 0.003 | 0.020 ± 0.002 | 0.022 ± 0.003 | 0.019 ± 0.003 | 0.017 ± 0.001* | 0.025 ± 0.003 | 0.021 ± 0.004 | 0.020 ± 0.004 | 0.023 ± 0.004 | 0.022 ± 0.003 | 0.017 ± 0.003 | |
Testis(Rt.)/ Ovary (Lt.) | Male | 3.391 ± 0.243 | 3.669 ± 0.398 | 3.767 ± 0.306 | 3.607 ± 0.142 | 3.606 ± 0.788 | 3.666 ± 0.315 | 3.324 ± 0.689 | 3.831 ± 0.472 | 3.822 ± 0.331 | 3.594 ± 0.362 | 3.632 ± 0.851 | 4.047 ± 0.343 |
Female | 3.594 ± 0.362 | 0.146 ± 0.030 | 0.164 ± 0.049 | 0.145 ± 0.017 | 0.157 ± 0.038 | 0.157 ± 0.047 | 0.149 ± 0.017 | 0.156 ± 0.022 | 0.152 ± 0.016 | 0.148 ± 0.018 | 0.166 ± 0.036 | 0.164 ± 0.017 | |
% to body weight | Male | 0.698 ± 0.052 | 0.610 ± 0.054 | 0.593 ± 0.050 | 0.716 ± 0.109 | 0.567 ± 0.109 | 0.548 ± 0.033 | 0.684 ± 0.095 | 0.613 ± 0.079 | 0.579 ± 0.032 | 0.751 ± 0.114 | 0.571 ± 0.128 | 0.587 ± 0.046 |
Female | 0.046 ± 0.007 | 0.044 ± 0.010 | 0.048 ± 0.014 | 0.051 ± 0.005* | 0.048 ± 0.013 | 0.049 ± 0.015 | 0.056 ± 0.007* | 0.051 ± 0.006 | 0.048 ± 0.006 | 0.055 ± 0.002 | 0.052 ± 0.010 | 0.049 ± 0.006 | |
Epididymis (Lt.)/Uterus | Male | 1.264 ± 0.099 | 1.542 ± 0.205 | 1.555 ± 0.142 | 1.440 ± 0.207 | 1.547 ± 0.329 | 1.562 ± 0.403 | 1.322 ± 0.170 | 1.550 ± 0.306 | 1.575 ± 0.170 | 1.431 ± 0.080* | 1.511 ± 0.271 | 1.669 ± 0.262 |
Female | 0.649 ± 0.173 | 0.803 ± 0.289 | 0.883 ± 0.114 | 0.879 ± 0.283 | 0.991 ± 0.340 | 0.970 ± 0.274 | 0.715 ± 0.195 | 0.907 ± 0.213 | 0.691 ± 0.152 | 0.752 ± 0.338 | 0.770 ± 0.264 | 0.893 ± 0.365 | |
% to body weight | Male | 0.260 ± 0.021 | 0.260 ± 0.054 | 0.245 ± 0.026 | 0.285 ± 0.050 | 0.244 ± 0.047 | 0.231 ± 0.043 | 0.273 ± 0.015 | 0.248 ± 0.050 | 0.239 ± 0.032 | 0.299 ± 0.035 | 0.240 ± 0.053 | 0.241 ± 0.029 |
 | Female | 0.225 ± 0.070 | 0.245 ± 0.092 | 0.264 ± 0.048 | 0.315 ± 0.108 | 0.299 ± 0.102 | 0.296 ± 0.084 | 0.269 ± 0.074 | 0.298 ± 0.082 | 0.217 ± 0.044 | 0.272 ± 0.084 | 0.240 ± 0.082 | 0.271 ± 0.125 |