Figure 2
From: Water extract of Spatholobus suberectus inhibits osteoclast differentiation and bone resorption
Inhibitory effect of WESS on osteoclast differentiation in BMMs. BMMs were cultured with vehicle or WESS in the presence of M-CSF (60 ng/ml) and RANKL (100 ng/ml) for 4 days. (A) Representative microscopic pictures of TRAP staining. (B) Total cellular TRAP activity. (C) The number of TRAP(+)MNC containing three or more nuclei. (D) Effect of WESS on the viability of BMMs in the presence of M-CSF (60 ng/ml). All bar graphs represent mean ± SD of three independent experiments. b P < 0.01, c P < 0.001 vs. control group.