Gene expression analysis and enzymatic activity of osteoblastogenic markers, for gene expression, 24 h FCS deprived hFOB1.19 cells were cultured in the presence of sesamin at indicated concentrations for another 24 h. cDNA of each samples was analyzed by Real-time PCR. The expression of COL1, ALP, and BMP-2 (A) The expression of Runx2 and OC. (B) The expression of OPG and its counterpart RANKL (C) ALP activity was detected in culture media at days 3, 7 and 14 of culture using an alkaline phosphatase detection kit (D). All data are shown as mean ± S.D. of three independent experiments. Statistical analysis was analyzed by independent T-test, * and ** denoted for p-value ≤ 0.05 and 0.01, respectively.