Pro-apoptotic effects of saffron.(A) Annexin-PI measurements of untreated cells (Ctrl) and the two cell types, HCT116 p53 wildtype and HCT116 p53 −/− cells, treated with 3 mg/ml saffron for 24 and 48 h. The profile represents Annexin-V-FITC staining in x axis and PI in y axis. (B) Quantitative analysis of percentage gated for viable, necrotic, early apoptotic, and late apoptotic HCT116 p53 wildtype and HCT116 p53 −/− cells treated with 3 mg/ml saffron for 24 and 48 h. Results are means ± s.d. (n = 3). (*, Saffron treatment was statistically significant in HCT116 p53 wildtype against HCT116 p53 −/− cells for P < 0.05).