Baicalin up-regulates Foxp3 expression in CD4
T cells. ( A) FACS-sorted CD4+CD25- T cells from B6 mice were stimulated with indicated doses of Baicalin for 3 days. Foxp3 expression in the gate of CD4+ T cells was determined by flow cytometry. (B) CD4+CD25- T cells from B6 mice were cultured in the presence or absence of Baicalin for 3 days. Using confocal microscopy, nuclear Foxp3 expression was detected in the CD4+ T cells. The scale bar represents 4 μm. (C) Five independent cells were selected randomly from each sample, and shown are results from three independent experiments. ( D) CFSE labeled CD4+CD25- T cells were co-cultured with CD4+CD25+ T cells at a 2:1 ration in the presence or absence of Baicalin for 5 days, the proliferation of cells was determined by flow cytometry.