Baicalin up-regulates Foxp3 expression in HEK 293 T cells. Foxp3 mRNA levels in transfected HEK 293 T cells are shown ( A) Twenty-four hours after transfection, cells were treated with Baicalin for another 24 h. The dose-dependent induction of Foxp3 mRNA expression is shown. Date are expressed as mean ± SD and compared with the vector control (expression level of the vector control was set as 1.0). ( B) After transfection with Foxp3 expression plasmids, a second group of cells was treated with 20 μM Baicalin. The time-dependent induction of Foxp3 mRNA expression is shown. Data are expressed as mean ± SD (expression level at 0 h was set as 1.0). ( C) Transfected HEK 293 T cells were treated with indicated cytokines in the presence or absence of Baicalin for another 24 h. Foxp3 mRNA was examined by RT-PCR. ( D) HEK 293 T cells were treated with IL-6 in the presence or absence of Baicalin for 24 h, and IL-6R mRNA levels were examined by RT-PCR.