Percent effect of VBME (100, 200 and 300mg/kg) and Tramadol
(30mg/kg) on tail immersion pain in mice. Each point represents the mean ± SEM of 6 animals. The data was analyzed by ANOVA followed by Dunnett’s test. Asterisks indicated statistically significant values from control. *P < 0.05, **P < 0.01, ***P < 0.001.