Percent effect of VBME (200 and 300mg/kg) and Tramadol
(30mg/kg) antagonized with naloxone (0.5mg/kg) on hot plat pain model in mice. Each point represent the mean ± SEM of six animals. The data was analyzed by ANOVA followed by Dunnett’s test. Asterisks indicated statistically significant values from control. *P < 0.05, **P < 0.01, **P < 0.001.