Figure 6

Dose dependent regulation of CCL2 expression by Tan IIA. (A) Regulation of CCL2 mRNA expression analyzed by RT-qPCR experiments. U-937 cells were treated as described in methods. Transcript levels were measured by RT-qPCR using BAL as an internal reference. Bars denote the standard error of the mean. All experiments were repeated three times. (B) Regulation of CCL2 protein expression determined by ELISA. U-937 cells were treated as described in methods. Experiments were repeated three times. Bars denote the standard error of the mean.