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Table 5 The weight and character of the pooled active fractions derived from the Sephadex LH-20 size exclusion chromatography of the bee pollen derived CDEb-5 fraction

From: Chemical constituents and free radical scavenging activity of corn pollen collected from Apis mellifera hives compared to floral corn pollen at Nan, Thailand

Fraction

Weight (mg)

Yielda

(CDEb-5)

Yieldb

(Bee pollen)

Character

Activityc

CDEb5-1

28.1

3.62

0.01

Brown solid

 

CDEb5-2

188.4

24.3

0.04

Dark brown solid

 

CDEb5-3

7.8

1.01

0.00

Dark brown solid

 

CDEb5-4

3.9

0.50

0.00

Brown solid

 

CDEb5-5

2.8

0.36

0.00

Brown solid

++

CDEb5-6

9.9

1.28

0.00

Yellow-green solid

++

  1. a,bYield as a % of the aCDEb5 fraction and bof the bee pollen
  2. cQualitative free radical scavenging activity, as determined by the DPPH assay sprayed onto the TLC plates, where -, +, ++ and +++ represent no, weak, moderate and strong color change (activity).