Induction of morphological changes and cytotoxicity in K562 cells by C. spinosa fractions without affecting normal human cells. K562 (A, B, C), Fibroblasts (D), or PBMCs (E) cells were treated with C.spinosa fractions at different concentrations for 48 h. Morphological changes were evaluated by direct observation using a light microscope at a magnification of 40X (B: 1. Ethanol; 2. S2Et; 3. P2Et; and 4. Etoposide). Cell cytotoxicity was estimated using the MTT assay (C, D, and E). Ethanol and etoposide were used as negative and positive controls, respectively. The results of six experiments are shown.