Effect of CAF on triglyceride accumulation and lypolytic activity in 3T3-L1 adipocytes. Confluent 3T3-L1 preadipocytes were differentiated into adipocytes in DMII medium for 6 days. (A) CAF reduced TG content during differentiation of 3T3-L1 cells. 3T3-L1 preadipocytes were differentiated in the absence or presence of CAF for 4 or 6 days, and the lipid accumulation was measured by triglyceride assay. Data are mean ± SD values of at least three independent experiments. (*) p < 0.05, (**) P < 0.01 compared with the differentiated adipocytes (control). (B) CAF increased lipolytic activity in 3T3-L1 adipocytes. The lypolytic activity of CAF was determined by measuring glycerol levels secreted in medium. All experiments were performed on triplicates for each treatment. Values represent mean ± SD. (*) p < 0.05, (**) P < 0.01 compared with the untreated adipocytes (control).