Effect of CAF on lipid accumulation of 3T3-L1 adipocytes. Confluent 3T3-L1 preadipocytes differentiated into adipocytes in medium containing different concentrations of CAF for 6 days (from day 0 to 6). Con, 3T3-L1 preadipocytes; DMII, fully differentiated adipocytes; 10 μg/ml, fully differentiated adipocytes (DMII + 10 μg/ml CAF); 50 μg/ml, fully differentiated adipocytes (DMII + 50 μg/ml CAF). (A, B) Inhibitory effects of CAF on lipid accumulation in 3T3-L1 adipocytes. The intracellular lipid accumulation quantified by Oil-red O staining and also optically observed by an inverted microscope. Oil-red O staining assay for lipid droplets was performed on day 6 after induction of differentiation. (C) Effect of CAF on cell viability in preadipocyte and differentiated adipocytes. These data were presented as relative cell viability values. Data are mean ± SD values of at least three independent experiments. Each experiment was performed in triplicate.