In vitro rate of 2-deoxyglucose uptake and GLUT-4 protein content in the soleus muscles of sham operated control (SHAM) and ovariectomized (OVX) rats treated with or without C. comosa hexane extract or compound 049. Animals are as described in legend of Figure 2. (A) 2-deoxyglucose uptake in the absence (blank bar) and presence (filled bar) of insulin (2 mU/ml); (B) net increase above basal level for 2-deoxyglucose uptake due to insulin; (C) whole muscle GLUT-4 protein levels. GLUT-4 levels were normalized by GAPDH. Values are reported as the mean ± SE for 7-8 animals/group. *P < 0.05 vs. SHAM group; † P < 0.05 vs. OVX group.