Pro-oxidant activity of crude aqueous extract of P. indica root in HeLa cancer cells. Measurement of thiobarbituric acid reactive species (TBARS) during malondialdehyde peroxidation in HeLa cells treated with 0.5 mg/ml of crude aqueous extracts at 24-hr and 48-hr incubation respectively were performed as described in Methods. Each experiment was done in triplicate. The pro-oxidant activity of the crude aqueous extract of P. indica root was evident. Data are expressed as mean ± SD. *P < 0.01.