Glucan-rich polysaccharides from Pleurotus sajor-caju (Fr.) Singer prevents glucose intolerance, insulin resistance and inflammation in C57BL/6J mice fed a high-fat diet

Table 2 Genes investigated

No	Gene name and abbreviation	Assay ID	Accession number
1	Adiponectin	Mm 00456425_m1	NM_009605.4
2	Glucose transporter	Mm 00436615_m1	NM_009204.2
	(GLUT-4)
3	Retinol binding protein 4	Mm 00803266_m1	XM_993476
	(RBP-4)
4	Nuclear factor-κB	Mm 00482418_m1	NM_003998
	(NF-κB)
5	Tumor necrosis factor-α	Mm 00443258_m1	NM_013693.2
	(TNF-α)
6	Serum amyloid A 2	Mm 00656927_g1	NM_009117.3
	(SAA-2)
7	Interleukin 6	Mm 00446190_m1	NM_031168.1
	(IL-6)
8	Monocyte chemoattractant protein-1	Mm 00437433_m1	NM_011331.2
	(MCP-1)
9	C-reactive proteins	Mm 00432680_g1	NM_007768.4
	(CRP)

General abbreviation of genes selected for this study and corresponding assay ID and accession number was obtained from the Applied Biosystems website and NCBI database. Assay ID refers to the Applied Biosystems Gene Expression Assays inventoried kits with proprietary primer and TaqMan® probe mix. Assay ID with ‘Mm’ is referred to as ‘Mus musculus’. All Gene Expression Assay kits indicated are FAM/MGB probed.

ISSN: 2662-7671