Effects of SCTE on the recruitment of leukocytes (A), mucus production (B), scoring of the extent of inflammation via quantitative analysis of inflammatory cell infiltration (C), scoring of mucus production (D) in lung tissue. Histological examination of lung tissues was performed 48 h after the final OVA challenge. Lung tissues were fixed, sectioned at 4 μm thickness, and stained with H&E (A) solution or PAS (B). PBS/PBS, PBS-sensitized/challenged, negative control (PBS only); OVA/PBS, OVA-sensitized/challenged mice, (PBS only); OVA/mon, OVA-sensitized/challenged mice (montelukast 30 mg/kg); OVA/SCTE-200, OVA-sensitized/challenged mice (SCTE 200 mg/kg); OVA/SCTE-400, OVA-sensitized/challenged mice (SCTE 400 mg/kg). SCTE or montelukast was given 1 h before the challenge. Significantly different from PBS/PBS, ##
P < 0.01; significantly different from OVA, *
P < 0.05, **
P < 0.01.