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Table 1 Primer Design for Quantitative RT-PCR Analysis

From: Standardized butanol fraction of WIN-34B suppresses cartilage destruction via inhibited production of matrix metalloproteinase and inflammatory mediator in osteoarthritis human cartilage explants culture and chondrocytes

mRNA Primersa Annealing Tm (cycle)
Aggrecan Fw: 5-TGAGGAGGGCTGGAACAAGTACC-3 58°C (32)
Rv: 5-GGAGGTGGTAATTGCAGG GAACA-3
Col II Fw: 5-AACACTGCCAACGTCCAGAT-3’ 58°C (32)
Rv: 5-CTGACGCACGGTATAGGTGA-3’
ADAMTS-4 Fw: 5’-GTCTGTGTC CAGGGCCGATGC-3’ 55°C (32)
Rv: 5’-GCCGCCGAAGGATCTCCAGAA-3’
ADAMTS-5 Fw: 5’-GCGGATGTGTGCAAGCTGACC-3’ 55°C (32)
Rv: 5’-AGTAGCC CATGCCATGCAGGA-3’
MMP-1 Fw: 5’-TCAGTTCGTCCTCACTCCAG-3’ 58°C (32)
Rv: 5’-TTGGTCCACCTGTCATCTTC-3’
MMP-3 Fw: 5’-ATGGACCTTCTTCAGCAA-3’ 58°C (32)
Rv: 5’-TCATTATGTCAGCCTCTC-3’
MMP-13 Fw: 5’-AGGAGCATGGCGACTTCTAC-3’ 58°C (32)
Rv: 5’-TAA AAA CAG CTC CGC ATC AA-3’
TIMP-1 Fw: 5’-GCA ACTCCGACCTTGTCATC-3’ 58°C (32)
Rv: 5’-AGCGTAGGTCTTGGTGAAGC-3’
TIMP-3 Fw:5’-GGCGGCAGCAGCGGCAATGAC-3’ 58°C (32)
Rv:5’-TACCAGCTTCTTCCCCACCACCTT-3’
β-actin Fw: 5’-GCTCTCCAGAACATCACTCCTGCC-3’ 58°C (27)
  Rv: 5’-CGTTGTCATACCAGGAAATGAGCTT-3’
  1. aFw, forward; Rv, reverse; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Tm, temperature