Table 1 Primer Design for Quantitative RT-PCR Analysis
mRNA | Primersa | Annealing Tm (cycle) |
|---|---|---|
Aggrecan | Fw: 5′-TGAGGAGGGCTGGAACAAGTACC-3′ | 58°C (32) |
Rv: 5′-GGAGGTGGTAATTGCAGG GAACA-3′ | ||
Col II | Fw: 5′-AACACTGCCAACGTCCAGAT-3’ | 58°C (32) |
Rv: 5′-CTGACGCACGGTATAGGTGA-3’ | ||
ADAMTS-4 | Fw: 5’-GTCTGTGTC CAGGGCCGATGC-3’ | 55°C (32) |
Rv: 5’-GCCGCCGAAGGATCTCCAGAA-3’ | ||
ADAMTS-5 | Fw: 5’-GCGGATGTGTGCAAGCTGACC-3’ | 55°C (32) |
Rv: 5’-AGTAGCC CATGCCATGCAGGA-3’ | ||
MMP-1 | Fw: 5’-TCAGTTCGTCCTCACTCCAG-3’ | 58°C (32) |
Rv: 5’-TTGGTCCACCTGTCATCTTC-3’ | ||
MMP-3 | Fw: 5’-ATGGACCTTCTTCAGCAA-3’ | 58°C (32) |
Rv: 5’-TCATTATGTCAGCCTCTC-3’ | ||
MMP-13 | Fw: 5’-AGGAGCATGGCGACTTCTAC-3’ | 58°C (32) |
Rv: 5’-TAA AAA CAG CTC CGC ATC AA-3’ | ||
TIMP-1 | Fw: 5’-GCA ACTCCGACCTTGTCATC-3’ | 58°C (32) |
Rv: 5’-AGCGTAGGTCTTGGTGAAGC-3’ | ||
TIMP-3 | Fw:5’-GGCGGCAGCAGCGGCAATGAC-3’ | 58°C (32) |
Rv:5’-TACCAGCTTCTTCCCCACCACCTT-3’ | ||
β-actin | Fw: 5’-GCTCTCCAGAACATCACTCCTGCC-3’ | 58°C (27) |
|  | Rv: 5’-CGTTGTCATACCAGGAAATGAGCTT-3’ | |