Figure 4

Effect of CTC on LPS-induced iNOS and COX-2 protein levels in RAW 264.7 cells. (A) Cells were treated with different concentrations of CTC (125, 250, 500 μg/ml) for 1 h and then with LPS (10 μg/ml) for 24 h. β-actin expression is shown as a loading control. (B) Relative expression of iNOS and COX-2. Bands on western blots were quantified by densitometry. The values are expressed as the means ± SD of at least three independent experiments (^# p < 0.05 vs. the non-treated control group; *p < 0.05, **p < 0.01 vs. the LPS-treated group).