Figure 3

Detection of morphological changes related to apoptosis and DNA fragmentation analysis. Data are representative one of three similar experiments. Induction of decoction on morphological changes, apoptotic bodies formation and DNA fragmentation in HepG2 cells. The cells were treated with indicated dose of decoction for 24 h and (A) anti-proliferative effect on PBM cells and HepG2 cells (B) photographed by phase contrast microscope. (C) The cells were stained with acridine orange/ethidium bromide and visualized for nuclear morphology and apoptotic bodies under fluorescent microscope using a blue filter (D) The cells were stained with Hoechst 33258 and visualized for nuclear morphology and apoptotic bodies under fluorescent microscope using a blue filter (E) Fragmented DNA was extracted and analyzed on 2% agarose gel electrophoresis.