The effect of DG on mitochondrial depolarization in H/R-challenged H9c2 cells. After H/R was challenged in the presence or absence of DG (125 and 250 μg/ml) or trolox (20 μM), mitochondrial depolorization was measured by flow cytometry with JC-1 staining. The data were expressed as mean ± S.E.M. Three independent experiments were performed. ###p < 0.001 indicated the groups with significant difference when compared to the normoxic group. **p < 0.01 and ***p < 0.001 when compared to the control group.