The effect of DG on apoptotic protein expression in H/R-challenged H9c2 cells. After treatment with H/R in the presence or absence of DG, A) Caspase 3 and bcl 2 expression in total cellular protein extract was detected by Western blot which was normalized by β-actin. B) Cytochrome c expression in cytosolic fraction (normalized by β-actin) and mitochondrial fraction (normalized by COX-IV) were detected by Western blot. Relative band intensity was calculated by dividing the intensity of each treated sample by that of the control group. The data were expressed as mean ± S.E.M. The figure is a representative of three individual experiments. The data were expressed as mean ± S.E.M. #p < 0.05 and ##p < 0.01 indicated the groups with significant difference when compared to the normoxic group. *p < 0.05 and **p < 0.01 when compared to the control group.