Leaf extract of Sarracenia purpurea prevents high glucose-mediated cytotoxicity. (A) Exposure to glucose toxicity elicited a significant loss in cell survival compared to normal glucose conditions as assessed by Live/Dead assay [left panel, ** denotes a significant difference (p < 0.01) relative to normal glucose control, Student’s t-test, n = 8]. Multiple concentrations of leaf extract were administered to high glucose-treated cells to confirm cytoprotection [right panel, # and ## denote significant differences (p < 0.05 and ## p < 0.01, respectively) relative to high glucose control, ANOVA, post-hoc Dunnett’s t-test, n = 8]. (B) Treatment with 25 μg/mL of leaf extract reversed high glucose-induced loss of PC12 cells, which were identified by intracellular cleavage of calcein-AM to its fluorescent product (green cells) without loss of membrane integrity (red cells). Data are reported as the mean ± SEM.