Effect of CG on phosophorylation of Akt and GSK3β during 3T3-L1 differentiation. 3T3-L1 preadipocytes were differentiated in the absence or in the presence of CG for 4 or 6 days. (A, B) 3T3-L1 adipocytes were treated with CG for indicated concentration and phosphorylation levels for Akt and GSK3β were determined by Western blotting analysis. The extent of phosphorylation was calculated by determining the amount of phosphorylated Akt relative to total Akt levels (DMII or DMII + 100 μg/ml CG). Data are mean ± SD values of at least three independent experiments. (*) p < 0.05, (**) P < 0.01.