Effect of SG on the activation of ER α, ERK and Akt in the MCF-7 cells. Total and phosphorylation state of each protein was measured in MCF-7 cells which were treated with SG at 1 μg/ml for the indicated time. The whole cell lysates were immunoblotted with anti-p- ERα/ERK/Akt or ERα/ERK/Akt antibody. The intensity of the phosphorylated or total ERα/ERK/Akt was quantified by densitometry, and the intensity of the phosphorylated ERα/ERK/Akt band was normalized to that of total ERα/ERK/Akt. *P < 0.05 versus control.