Figure 3
ACA inhibits p65 (RelA) nuclear retention in HSC-4 human oral cancer cells. (A) Western blot analysis on p65 localization levels indicated that cytoplasmic p65 increased, while nuclear p65 decreased upon ACA exposure for 4 h. (B) All band intensities between cytoplasmic and nuclear extracts were quantified and normalized against β-actin and histone H3 respectively, using the ImageJ v1.43u software.