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Figure 2 | BMC Complementary and Alternative Medicine

Figure 2

From: 1’-Acetoxychavicol acetate inhibits growth of human oral carcinoma xenograft in mice and potentiates cisplatin effect via proinflammatory microenvironment alterations

Figure 2

The effects of ACA on the migration and apoptotic properties of HSC-4 human oral carcinoma cells. (A) Inhibition on migration rate by ACA as demonstrated using the wound healing assay with DMSO as a solvent control. (B) Quantification on open wound areas after 24 h of incubation using the TScratch software. (C) Induction of apoptosis by ACA through activation of caspase-3 and subsequent cleavage of full length PARP enzymes (116 kDa) into a small (24 kDa) and a large (89 kDa) subunit protein, with β-actin as a loading control. (D) Confirmation of apoptosis-mediated cell death in HSC-4 cells through the visualization of 150 to 200 bps laddering of genomic DNA upon ACA treatment as demonstrated by DNA fragmentation assay.

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