Curcumin inhibits connective tissue growth factor (CTGF) expression in liver tissue of CCl
treated rats. (A) Immunohistochemical staining for CTGF in rat liver (original magnification, ×200). (B) Western blots analysis of hepatic CTGF expression and GAPDH as loading control. Blots obtained from several experiments were analysed using densitometry, and the densitometric values were pooled from four animals per group and are shown as means ± SD in the bar graph. *P < 0.05 for Cur/CCl4 vs. PBS/CCl4. (C) The levels of CTGF mRNA in liver tissue were determined by quantitative real-time RT-PCR and normalized relative to GAPDH mRNA expression in each sample, with values expressed as the mean ± SD fold increase over normal control group (n=6). *P < 0.01 for Cur/CCl4 vs. PBS/CCl4.