Effect of ARA extract on GLUT4 expression in 3T3-L1 cells. The cells were induced to differentiate into adipocytes with MDI medium with or without ARA (100, 200 and 500 mg/mL) extract for 8 days. (A) The expression of GLUT4 protein was analyzed by western blot. (B) Representative image of GLUT4-positive cells by immunostaining. Left panels, GLUT4-positive staining; middle panels, nuclear DAPI staining; and right panels, merged images of GLUT4 and DAPI. P: preadipocyte, C: control, 100: ARA 100 μg/mL, 250: ARA 250 μg/mL and 500: ARA 500 μg/mL.