Effect of ARA extract on PPARγ expression in 3T3-L1 cells. The cells were induced to differentiate into adipocytes with MDI medium with or without ARA (100, 200 and 500 mg/mL) extract for 8 days. (A) The expression of PPARγ mRNA was analyzed by RT-PCR. Relative density was calculated as the ratio of PPARγ expression to GAPDH expression. (B) The protein expression of PPARγ was analyzed by western blot. Relative density was calculated as the ratio of PPARγ expression to actin. Each value represents the mean ± S.D. (n = 3). *p < 0.05 vs. control. P: preadipocyte, C: control, 100: ARA 100 μg/mL, 250: ARA 250 μg/mL and 500: ARA 500 μg/mL.