Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts

Table 3 Effect of D. gnidium extracts on the genotoxicity induced by nitrofurantoin (5 μg/assay)

Extracts	Doses (μg/assay)	β-gal (UE)	PA (UE)	IF	Inhibition of genotoxicity (%)
Nitrofurantoin	5	42,18 ± 0,092	11,19 ± 0,0021	23,56	-
NC	0	3,07 ± 0,024	18,58 ± 0,007	1	-
Petroleum ether extract	50	26,21 ± 0,011	9,82 ± 0,019	16,68	30,50*
	250	26,65 ± 0,041	9,61 ± 0,011	17,31	27,70*
	500	27,5 ± 0,019	9,87 ± 0,007	17,37	27,43*
Chloroform extract	50	14,31 ± 0	7,05 ± 0	12,29	49,90**
	250	16,18 ± 0,0169	10,29 ± 0	9,53	62,19**
	500	4,66 ± 0,047	4,45 ± 0	6,35	76,28**
Ethyl acetate extract	5	8,19 ± 0,019	5,21 ± 0	9,53	62,18**
	15	7,18 ± 0,0183	5,29 ± 0,0028	8,23	67,95**
	30	7,93 ± 0,0127	5,68 ± 0,007	8,46	66,93**
Methanol extract	50	14,67 ± 0,001	9,48 ± 0,08	9,38	62,85**
	100	15,98 ± 0,08	10,55 ± 0,04	9,18	63,74**
	200	15,42 ± 0,004	12,464 ± 0,042	7,50	71,18**
TOF extract	5	19,73 ± 0,014	8,41 ± 0,009	14,66	39,45*
	15	17,68 ± 0,029	7,04 ± 0,007	15,68	34,92*
	30	14,76 ± 0,009	7,17 ± 0,004	12,87	47,38*

β-gal: units of β-galactosidase; AP: units of phosphatase alkaline; IF: induction factor.
Positive control of genotoxicity (nitrofurantoin); NC: negative control (non-treated cells).
Results were expressed as percentage of inhibition of genotoxicity compared to the positif control (Nitrofurantoin). Values represent the mean ± SD of three separate experiments. The statistical significance of results was evaluated by the Student’s t-test. *P < 0.05, **P < 0.01 means significant difference between positif control and treated sample.

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