A, B and C: The RANKL, OPG, M-CSF gene expressions for all the groups. Samples were normalized to GAPD MFI (Median Fluorescence Intensity). Error bars represented the standard deviations of the average responses. Significant difference was taken at p < 0.05. SHAM : Sham- operated group. ORX : Orchidectomized control group. ORX + T : Orchidectomized group treated by testosterone. ORX + EL : Orchidetctomized group treated with EL.