Figure 4

Aggregation assays were performed using a protein concentration of 10 μg of native PrTX-III or PrTX-III previously treated with harpalycin 2 (HP-2), aristolochic acid (Aris Ac) or p-bromophenacyl bromide (p-BPB) (a). The effect of previous incubation of platelets with HP-2 before the administration of PrTX-III or arachidonic acid is showed in (c). Previous incubation with AACOCF3 or INDO before the administration of PrTX-III is showed in (e). (b), (d) and (f) demonstrate the platelet aggregation results after 8 minutes of experimental condition and each point in the respective table represent the mean ± SEM (n = 4) and *p < 0.05.