Effects of GS on DOX-induced apoptosis-related protein in the cytoplasm of H9C2 cells. (A) Cells (5 × 105 cells/ well) were pretreated with the indicated concentrations of GS for 2 h before incubation with DOX (1 μM) for 24 h. The proteins (30 μg) of total cell lysates were analyzed at the expression levels of anti-PARP, caspase-3, bcl-2, bax, and cytosolic lysates that were analyzed for anti-cytochrome C by Western blotting at 10% SDS-PAGE. β-Actin is the internal standard to confirm equal loading. (B) Quantification of PARP, bcl-2, and caspase-3 expression, and cytochrome C release. (C) Quantification of the cleaved form of PARP and caspase-3, as well as the bax expression. Data are expressed as mean ± S.E. from 3 independent experiments. *P < 0.05, **P < 0.01, and PARP (both the precursor and cleaved form) expression compared to the DOX treatment alone; #P < 0.05 and ##P < 0.01, and caspase-3 (both the precursor and cleaved form) compared to the DOX treatment alone; ςP < 0.05 and ςςP < 0.01, and bcl-2 and bax compared to the DOX treatment alone. ξP < 0.05 and ξξP < 0.01, and cytochrome C compared to the DOX treatment alone.