GS prevention on DOX-induced H9C2 cell death. Cells were incubated with DOX (1 μM) in the presence and absence of GS for 24 h. The percentages of viable cells were determined using an MTT assay, as described in the Materials and Methods section. The results are expressed as mean±S.E. from 3 independent experiments. *P < 0.05 and **P < 0.01 indicate a significant difference from DOX treatment. #P < 0.01 indicates a significant difference from the control (GS 0 μM and DOX 0 μM).