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Table 2 Treatment of K562 cell DNA with Isorhamnetin 3- O -robinobioside (I3- O - Rob) and ethyl acetate extract (EA extract) in the presence and abscence of H 2 O 2 in comet test

From: Isorhamnetin 3-O-robinobioside from Nitraria retusa leaves enhance antioxidant and antigenotoxic activity in human chronic myelogenous leukemia cell line K562

Comet assay on genomic DNA of K562 cells treated with Isorhamnetin 3- O -robinobioside (I3- O - Rob) and ethyl acetate extract (EA extract) Inhibitory effect of Isorhamnetin 3- O -robinobioside (I3- O - Rob) and ethyl acetate extract (EA extract) on the genotoxicity of K562 cells against H2O2
Extracts Concentrations μg/ml) Total DNA Damage (TDD) Total DNA Damage (TDD) Inhibition percentage (%)
T - 252 ± 6 252 ± 6  
H2O2 75 μM 360 ± 5 360 ± 5  
Ethyl Acetate extract 200 253 ± 5 298 ± 4 57.40*
400 261 ± 4 287 ± 5 67.6*
800 257 ± 6 276 ± 4 77.77*
Isorhamnetin 3-O-robinobioside 250 260 ± 5 297 ± 6 58.33*
500 255 ± 4 286 ± 5 68.51*
1000 254 ± 4 273 ± 5 80.55*
  1. * P < 0.05 compared to negative control without Isorhamnetin 3-O-robinobioside (I3- O- Rob) and ethyl acetate extract (EA extract) by student test. (results are represented by the means ± SD of three experiments).