Isorhamnetin 3-O-robinobioside from Nitraria retusa leaves enhance antioxidant and antigenotoxic activity in human chronic myelogenous leukemia cell line K562

Table 2 Treatment of K562 cell DNA with Isorhamnetin 3- O -robinobioside (I3- O - Rob) and ethyl acetate extract (EA extract) in the presence and abscence of H ₂ O ₂ in comet test

Comet assay on genomic DNA of K562 cells treated with Isorhamnetin 3- O -robinobioside (I3- O - Rob) and ethyl acetate extract (EA extract)			Inhibitory effect of Isorhamnetin 3- O -robinobioside (I3- O - Rob) and ethyl acetate extract (EA extract) on the genotoxicity of K562 cells against H₂O₂
Extracts	Concentrations μg/ml)	Total DNA Damage (TDD)	Total DNA Damage (TDD)	Inhibition percentage (%)
T	-	252 ± 6	252 ± 6
H₂O₂	75 μM	360 ± 5	360 ± 5
Ethyl Acetate extract	200	253 ± 5	298 ± 4	57.40*
	400	261 ± 4	287 ± 5	67.6*
	800	257 ± 6	276 ± 4	77.77*
Isorhamnetin 3-O-robinobioside	250	260 ± 5	297 ± 6	58.33*
	500	255 ± 4	286 ± 5	68.51*
	1000	254 ± 4	273 ± 5	80.55*

* P < 0.05 compared to negative control without Isorhamnetin 3-O-robinobioside (I3- O- Rob) and ethyl acetate extract (EA extract) by student test. (results are represented by the means ± SD of three experiments).

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